depends on what kind of algae you have on your glass. Oto's only eat certain kinds. It would probably be a good idea to do some research on what kind of algae it is and if oto's eat it. But, with a well established 3 year old tank, if you supplement with zucchini a couple times a week, 3 or 4 of them would probably be just fine in your tank. Make sure you buy healthy oto's, check to see if their bellies are nice and rounded or sunken in. Don't buy them if they look starved; they probably won't live long. Good luck!