if you are talking about bio-spira I tried it while cycling my 29 gal. I tried to be patient and wait the whole time, testing my water daily, but then got tired of it and heard about bio spira, so i found some at the LFS, used it and tested my water the nextday with fish in it ( put fish in per directions..I think...been a while) and the water seemed to have jumped drasticly as far as nitrate/nitrite/amonina levels (good direction) still wasnt what most people would say is healthy for the fish, but all of mine survived and are still here...yeah..
thats my story Hope you are talking about same stuff.