I attempted a fishless cycle using 5ppm of ammonia, gravel from an established tank and bio-spira. When I saw that ammonia had dropped from 5ppm to 3, I added most of the bio-spira. The next day, I had 3ppm nitrites in the tank and 3ppm ammonia and like 10ppm nitrates. Okay, good, right? I added the rest of the bio-spira since the nitrite eaters now had something to eat. I tested every day for two weeks and nothing changed. Now, I don't know if the bio-spira was bad. My gut says yes but I don't know enough about water chemistry to know for sure.
I do recall reading that the nitrogen cycle consumes KH. I only had like 4 degrees KH but I was told that was fine. I also noticed that the pH went up from ~7 to ~8. I'm guessing that that was due to the levels of pure ammonia in the tank. I don't know if those levels affected the process but, for whatever reason, the cycle stalled and bio-spira was of no help.
I e-mailed the company that makes it and they said that bio-spira needs fish in the tank to work................not sure why because fish produce ammonia and I merely added it to the tank...................?
Anyway, that's my experience with it. IMO, it's not worth the money. Since it seems that you have an established filter, I would take a pad from the filter (I think you said you have a canister?) and add it to your new one on the new tank and then add your fish. Boom, instant cycle. If you're worried about losing the bacteria from your old filter, I'm sure you could cut the old pad in half, add half a new pad to fill the space in the old filter and put the other halves of the new and old pads into the new filter to start your cycle?