This information was provided by one of our members over at MFK (RD.) and thought is was worth sharing with our AC members. A group of us did some research and testing the results were very positive. One of the products we used was Rid-X powder for septic systems. If one of the Mods would like to stick this it would be great. I hope you find this information as interesting as we did.
The use of probiotics in aquaculture has been around for decades but has gained some new popularity in the past few years due to a few fish food manufacturers incorporating some of these bacteria in their food.
While numerous publications & studies about the use of probiotics and/or prebiotics in aquaculture have emerged during the last decade, we still know very little about their use regarding the vast amount of ornamental species of fish found in this hobby.
Here's what we do know.
There are numerous strains of probiotic bacteria, each targeting different types of aquatic pathogens. In order for any of these probiotics to be fully effective, they must be in a live form, or able to be reactivated once in the GI tract of the host. One can also simply add these bacteria to the tank water on a regular basis.
http://www.keetonaqua.com/case-studies/tilapia/
http://www.sciencedaily.com/releases...1203163534.htm
Probiotics have been applied as dietary additives or water additives to improve growth performance and immune response, to improve water quality (by increased waste reduction), and to outcompete pathogenic bacteria.
Bacillus species such as B. subtilis and B. licheniformis are commonly used as probiotics in aquaculture, but there are numerous other bacteria species that are also being utilized within the aquatic industry. In order for probiotics to have any type of positive effect on the fish, they must survive in very large quantity.
Probiotics are usually defined as live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance (Fuller, 1989). Based on this definition, probiotics may include microbial adjuncts that prevent pathogens from proliferating in the intestinal tract (Gatesoupe, 1994). Most probiotics proposed as biological control agents in aquaculture belong to the lactic acid bacteria(LAB). But LAB has some limitations due to having a small antibacterial spectrum. These activities normally inhibit only closely related species of gram-positive microorganisms (Suma et al., 1998). However, almost all the pathogens involved in aquaculture are gram-negative bacteria. Bacillus subtilis, a gram-positive, aerobic, endospore-forming bacterium, would fall under this category, yet this is exactly what two of the fish food manufacturers using probiotics have listed in their ingredients as their only source of probiotic bacteria. The reason for that seems to be that spores of Bacillus strains are thermo-stabile which makes them much easier to use in heat processed pellets.
This is certainly not a new concept in aquaculture, these same type of heterotrophic bacteria have been manufactured for use in septic systems for decades, and there are a number of aquatic related companies that have been marketing these same types of enzymes & micro organisms for just as long.
A couple of fish food manufacturers have stated that their probiotic is based on Bacillus subtilis spores, a good commercial example of that being Calsporin, manufactured by the Calpis Co. Ltd out of Tokyo Japan.
Does it work? Well, according to the more positive studies, yes & no.
http://munin.uit.no/bitstream/handle...pdf?sequence=1
While it does improve the overall feed conversion ratio (FCR) the food used in that study mostly consisted of soybeans & wheat, and while it did have a positive affect on the intestinal microbiota at the initial stages of the feed trial, that apparently soon diminished over time. The longer it was fed, the lesser the positive affect. Also, while it was shown to elevate the expression of some immune related genes, it did not improve the disease resistance of koi challenged with Aeromonas hydrophila, which is one of the most problematic species of Aeromonas found in commercial aquaculture.
So what does that tell the average hobbyist that keeps ornamental species of fish?
Not a whole lot.
If you feed your fish a diet that is high in terrestrial based carbs such as gluten meal, corn, soybeans, wheat, potatoes, rice, etc - adding a probiotic such as Bacillus subtilis in large enough quantity could possibly increase the feed conversion ratio (depending on the species of fish), thereby causing an increase in growth, as well as a reduction in overall waste compared to feeding the same quality of feed, sans the probiotic. It could also help elevate the immune system of the fish, but so could numerous bioactive compounds found in natural raw ingredients such as, Antarctic Krill, Garlic, Spirulina, and micro algae such as Haematococcus pluvialis. Not to mention utilizing key vitamins & trace minerals at levels that far exceed the industry standards. As an example, instead of having a post extrusion level of 140 mg/kg of Vitamin C, having a level of 500+ mg/kg of Vitamin C. (with most of that coming from the raw ingredients themselves) Certainly some of these bacteria have been shown in some studies and using certain species of fish that they can improve the overall feed conversion ratio ....... but of foods that mostly consist of wheat & soybeans.
Of course that's a non issue for anyone that is NOT feeding large amounts of terrestrial based starch derived from corn, wheat, soybeans, potatos, etc to their fish.
Some of this is just plain old common sense, but some consumers can easily be sucked in by the hype.
Feed low cost crappy ingredients but add *special* bacteria to digest crappy ingredients, and charge twice the price of higher quality feeds that contain higher quality raw ingredients ....... simply because the former contain bacteria spores? Unfortunately many people buy into this type of hype or fad marketing.
The truth is you can have both, without breaking the bank on bacteria that comes from the dirt, and is dirt cheap if you know where to look (not at your LFS). More on that later.
Beyond just increasing the feed conversion ratio, and promoting growth, probiotics are also very effective at reducing waste. From an organic reduction standpoint, this isn't exactly cutting edge technology. These exact same heterotrophic bacteria have been used to digest & remove waste from septic systems for decades, the only difference being that in the past no one referred to them as probiotics. And as many of these septic system companies saw the potential for added revenue, they started marketing and selling these non-pathogenic *probiotic* bacteria for ponds, and aquariums. The truth is some are just as safe, and work just as well, as many of the aquatic based products that sell for 10-20 times the price. Some are the exact same bacteria being used, with a different label.
http://www.bio-cat.com/products
There's another plus side to dosing with these types of bacteria (non pathogenic) beyond just consuming organics, or increasing feed conversion ratios - it's called competitive exclusion.
http://www.theaquariumwiki.com/Competitive_Exclusion
While numerous publications & studies about the use of probiotics and/or prebiotics in aquaculture have emerged during the last decade, we still know very little about their use regarding the vast amount of ornamental species of fish found in this hobby.
Here's what we do know.
There are numerous strains of probiotic bacteria, each targeting different types of aquatic pathogens. In order for any of these probiotics to be fully effective, they must be in a live form, or able to be reactivated once in the GI tract of the host. One can also simply add these bacteria to the tank water on a regular basis.
http://www.keetonaqua.com/case-studies/tilapia/
http://www.sciencedaily.com/releases...1203163534.htm
Probiotics have been applied as dietary additives or water additives to improve growth performance and immune response, to improve water quality (by increased waste reduction), and to outcompete pathogenic bacteria.
Bacillus species such as B. subtilis and B. licheniformis are commonly used as probiotics in aquaculture, but there are numerous other bacteria species that are also being utilized within the aquatic industry. In order for probiotics to have any type of positive effect on the fish, they must survive in very large quantity.
Probiotics are usually defined as live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance (Fuller, 1989). Based on this definition, probiotics may include microbial adjuncts that prevent pathogens from proliferating in the intestinal tract (Gatesoupe, 1994). Most probiotics proposed as biological control agents in aquaculture belong to the lactic acid bacteria(LAB). But LAB has some limitations due to having a small antibacterial spectrum. These activities normally inhibit only closely related species of gram-positive microorganisms (Suma et al., 1998). However, almost all the pathogens involved in aquaculture are gram-negative bacteria. Bacillus subtilis, a gram-positive, aerobic, endospore-forming bacterium, would fall under this category, yet this is exactly what two of the fish food manufacturers using probiotics have listed in their ingredients as their only source of probiotic bacteria. The reason for that seems to be that spores of Bacillus strains are thermo-stabile which makes them much easier to use in heat processed pellets.
This is certainly not a new concept in aquaculture, these same type of heterotrophic bacteria have been manufactured for use in septic systems for decades, and there are a number of aquatic related companies that have been marketing these same types of enzymes & micro organisms for just as long.
A couple of fish food manufacturers have stated that their probiotic is based on Bacillus subtilis spores, a good commercial example of that being Calsporin, manufactured by the Calpis Co. Ltd out of Tokyo Japan.
Does it work? Well, according to the more positive studies, yes & no.
http://munin.uit.no/bitstream/handle...pdf?sequence=1
While it does improve the overall feed conversion ratio (FCR) the food used in that study mostly consisted of soybeans & wheat, and while it did have a positive affect on the intestinal microbiota at the initial stages of the feed trial, that apparently soon diminished over time. The longer it was fed, the lesser the positive affect. Also, while it was shown to elevate the expression of some immune related genes, it did not improve the disease resistance of koi challenged with Aeromonas hydrophila, which is one of the most problematic species of Aeromonas found in commercial aquaculture.
So what does that tell the average hobbyist that keeps ornamental species of fish?
Not a whole lot.
If you feed your fish a diet that is high in terrestrial based carbs such as gluten meal, corn, soybeans, wheat, potatoes, rice, etc - adding a probiotic such as Bacillus subtilis in large enough quantity could possibly increase the feed conversion ratio (depending on the species of fish), thereby causing an increase in growth, as well as a reduction in overall waste compared to feeding the same quality of feed, sans the probiotic. It could also help elevate the immune system of the fish, but so could numerous bioactive compounds found in natural raw ingredients such as, Antarctic Krill, Garlic, Spirulina, and micro algae such as Haematococcus pluvialis. Not to mention utilizing key vitamins & trace minerals at levels that far exceed the industry standards. As an example, instead of having a post extrusion level of 140 mg/kg of Vitamin C, having a level of 500+ mg/kg of Vitamin C. (with most of that coming from the raw ingredients themselves) Certainly some of these bacteria have been shown in some studies and using certain species of fish that they can improve the overall feed conversion ratio ....... but of foods that mostly consist of wheat & soybeans.
Of course that's a non issue for anyone that is NOT feeding large amounts of terrestrial based starch derived from corn, wheat, soybeans, potatos, etc to their fish.
Some of this is just plain old common sense, but some consumers can easily be sucked in by the hype.
Feed low cost crappy ingredients but add *special* bacteria to digest crappy ingredients, and charge twice the price of higher quality feeds that contain higher quality raw ingredients ....... simply because the former contain bacteria spores? Unfortunately many people buy into this type of hype or fad marketing.
The truth is you can have both, without breaking the bank on bacteria that comes from the dirt, and is dirt cheap if you know where to look (not at your LFS). More on that later.
Beyond just increasing the feed conversion ratio, and promoting growth, probiotics are also very effective at reducing waste. From an organic reduction standpoint, this isn't exactly cutting edge technology. These exact same heterotrophic bacteria have been used to digest & remove waste from septic systems for decades, the only difference being that in the past no one referred to them as probiotics. And as many of these septic system companies saw the potential for added revenue, they started marketing and selling these non-pathogenic *probiotic* bacteria for ponds, and aquariums. The truth is some are just as safe, and work just as well, as many of the aquatic based products that sell for 10-20 times the price. Some are the exact same bacteria being used, with a different label.
http://www.bio-cat.com/products
There's another plus side to dosing with these types of bacteria (non pathogenic) beyond just consuming organics, or increasing feed conversion ratios - it's called competitive exclusion.
http://www.theaquariumwiki.com/Competitive_Exclusion
