I recently tore my goldifish tank completely apart and scrubbed everything down rinsing the substrate in tapwater and even lifted the empty tank off the stand to wash it really well. My goldfish lived in a plastic bucket during the couple hours this clean took with their filter attached to the bucket. After I was done, I refilled the tank, waited for it to stabilize and re-added the fish. My ammonia and nitrites both remained at 0.0 since the bacteria in my biowheel did not get removed during this extreme cleaning of the tank.