okay, okay I give, I went out yesterday and bought some things for my tank. Sadly I lost my prized red gourami after I did a 25% water change. I bought some Start Right with Allantoin, and added it last night, and I bought some Stress Zyme and put that in. I found out that te bio filter was infact cleaned and removed of all life. So my apologies to wataugachicken, and everyone else. I hope now to save the rest of my fish and once it stabilizes I will purchase my red gourami again. My thanks to everyone who helped me, and put up with me.