UGF Setup

Water change will not affect the cycle. Provided you waited about an hour and had sufficeint filtration turnover, you won't affect the bio spira.
 
Thanks! That's a load off, I waited about 6 hours and now have ALOT of turnover. Still alot of gunk in the rocks though. I think I'll probably bug you in the future for RUGF advice...
 
Bio-spira is not going to work within hours not even over night! If your ammonia is high you need to do water changes until you get it down. Try not to over medicate your water, it can and usually will get you into more trouble than you already were in.
 
The bacteria in the biospira will very quickly attach to a hard surface and start multiplying there. Depending on a wide rage of variables the length of time it takes for them to multiply to the levels neccesarry to handle your bioload could take more then 24 hours, maybe even a day or two.

The bacteria that come in biospira will not live in the water column very long, so they quickly attach and start growing on every surface they come in contact with (like in your filter).

I presume the source where you got the biospira it came refrigerated and you kept it refrigerated from the time of purchase until you added it to your tank?
 
I presume then that the ammonia is from live fish?

at 4.0 that is too high and you need to do a serious water change..

bio spira works well but not at that high of ammonia..in essence it won't handle that quick enough to keep the fish safe.
 
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