Our Nitrate level was at 20 before we did a 25% water change. We did the change and the nitrate levels didn't budge, still the same. The ammonia and nitrite levels are still at 0. The only other thing that we did was add a carbon filter to take out rest of medication in the water. My husband said he did scrape off a lot of brown algae around the inside frame of the aquarium, trying to make sure that none of it got back into the water. If any of the algae did get back into the water, would that have anything to do with the nitrate reading??? I know that 20 isn't that big of a deal, but usually when we do a water change it will fall to 10. I tested our tap water and that comes in at 5. All fish are accounted for so what else could have made the change?