I am looking to doing the DIY CO2 again with a 48 gallon tank. Before I used 2 2L bottles with a T into a airstone. If I make a third bottle at the same time as the first two and just kept to sealed would it hurt anything?
Lol. How knows. But at that size of tank its not a good idea to use C02. I would use 4-5 bottles. Also dont use an airstone. It is not doing anything. Look for a "defusser".
I'd make up CO2 as you need it - never cap the mix of yeast, sugar and water in the hopes of storing it, CO2 will build up and cause it to explode, as Tanker said.
You can tell how much CO2 is in the tank by using KH and pH measurements and a CO2 chart. Chuck's planted aquaria site has lots of great tools for measuring ferts and CO2, including a calculator you can download.
I am looking to doing the DIY CO2 again with a 48 gallon tank. Before I used 2 2L bottles with a T into a airstone. If I make a third bottle at the same time as the first two and just kept to sealed would it hurt anything?
Dude you need to be seriously careful with that. Do not underestimate the amount of pressure that can be produced by yeast - it WILL make a 2 liter pop bottle explode. It would make one hell of a bang, and that icky sticky sugar/yeast mixture would splatter all over everything in the area like a dumptruck driving through a mudpuddle.
You should NEVER cap the opening of a DIY yeast CO2 bottle.