rrkss said:
Most enzymes have a certain pH range which they work best in. .
true.
You change the pH and you suddenly denature the protein somewhat (By affecting the hydrogen bonding that determines the protein's structure. .
you can not suddenly denature a protein somewhat. its complete, and often irreversibe, particularly if the tertiary activation apparatus is not on site (Golgi, if i remember right.)
This is similar to frying egg whites. What if the protein is used to transfer food across the cell membrane or assist in actively transporting toxic wastes out of the fish? By suddenly changing the pH, you suddenly change the fish's metabolism which could be very stressful.
i'm not following the egg white thing. yeah pH will effect protiens in the phospolipid bilayer, but only if the pH change is in the BLOOD. external pH change has to affect the pH of the blood for this to become a factor.
Rapid temperature changes have a similar effect. By gradually changing things, the fish have time to adjust to the changes by either switching proteins to ones optomized for that certain pH/temperature range or producing more repair proteins to assist in fixing the proteins as they denature. Normally a minor change like the one I suggested would not kill a fish but I asked the question since the fish was very ill and I wanted to get ideas of how to make this sudden environment change easier.
i would not throw temperature into the mix so readily, as that adds another variable to the equation entirely. agreed on the stress associated with denatured protiens, but again only if the change is in the blood, not the external envirnonments pH.
overall, i agree, and a valid concern with a sick fish. i would really like to see something clinical and/or laboratorical (word?) concerning pH changes on the fishes' ability to regulate blood pH, as that seems to be the real argument.
anyway, i hope your cherries recover. sounds like theyre in good hands.
eric