I would likely use filter media, not gravel... just because of the size of the bacterial colony.
if you do that, use as much as you can.
if you cannot transfer the media, just squeeze all the gunk and stuff out of it into your tank.
The bacteria colony will likely double each day or so, and if you feed sparingly, there will be less ammonia produced too.
but ultimately test your water to be sure, whatever you choose to do. and if there's enough bacteria being used to seed that will support the full fish load.. I'd likely add them all.
just feed sparingly, be ready to change water if necessary, and test as much as you can.. if you have 0 ammonia and nitrites with fish in the tank, you should be golden.