I run injected CO2 with a decent plant load and I had to do emergency aeration in the morning twice due to lack of oxygen. I now run injected CO2 when the lights are on on the same timer, then run a secound timer that goes on about 2 hours after lights/co2 off and runs til a half hour before lights on. yes, it wastes some co2, but its much better then gasping fish. Plants after lights out will use a decent amount of Oxygen as well, so its easy to deplete the levels, especially at warmer temps. I have the line to the diffusor T'ed, one leg runs from Co2 through a check valve tot he the T, the other is an airpump through a check valve to the T. The CO2 dissolves easily, but the air pump produces a decent amount of surface agitation. not as much as a direct airstone or HOB filter by any means, but enough to do the job and keep the oxygen level up through the night.