Acute toxicity and sublethal effects of ammonia and nitrite for juvenile cobia Rachycentron canadum
[SUP]a[/SUP] Ricardo V. Rodrigues a , Michael H. Schwarz [SUP]b[/SUP] , Brendan C. Delbos [SUP]b[/SUP] , Luı˙s A. Sampaio [SUP]a[/SUP],⁎
[SUP]a[/SUP] Fundação Universidade Federal do Rio Grande—FURG, Departamento de Oceanografia, Laboratório de Maricultura,
CP 474, Rio Grande, RS, 96201-900, Brazil
[SUP]b[/SUP] Virginia Tech—Virginia Seafood Agricultural Research and Extension Center, 102 S. King Street, Hampton, VA, 23669, USA
Received 7 February 2007; received in revised form 7 June 2007; accepted 13 June 2007
Cobia ceased to eat at 0.62 (0.56–0.70) ppm NH3-N and 76.1 (73.2–79.0) ppm NO2-N.
Swimming behavior was affected at higher concentrations: 0.80 (0.74–0.85) ppm NH3-N and 88.8 (82.6–95.5) ppm NO2-N. Even higher concentrations were necessary to kill juvenile cobia, LC50–96 h for ammonia was estimated at 1.13 (1.06–1.19) ppm NH3-N, and within the range of concentrations tested for nitrite it was not possible to estimate the LC50–96 h, as only 30% of the individuals died at the highest concentration after 96 h (210 ppm NO2-N). The results of the present experiments demonstrate that ammonia could be problematic at relatively low levels for the intensive rearing of juvenile cobia; however, it is unlikely that the high levels of nitrite needed to harm juvenile cobia would be reached in a well designed and properly operating RAS.