Since you already have the fish in the tank, you need to start very slowly with your injection.
Start in the morning at lights on. If you don't have a bubble counter or a reactor that you can count bubbles in, just take the end of the airline and stick it into the water and set the needle valve at one bubble every two seconds coming out of the end of the line. Then insert it into your reactor and wait 2hrs and test you pH to find CO2ppm.
You can then start to adjust your needle valve, in very small increments, to increase flow and thereby lower pH to the desired level. I would test every two hours or so, so that by lights out time you are no higher than 20ppm/CO2.
This will give you a cushion to keep the fish safe during lights out and you'll be able to see how much of a point drop your particular tank will get during the night. Then you can fine tune your flow from there.
I'm just very cautious with the fish in this regard. This is how I've started every one of my tanks, regardless of size, when first injecting and it's worked out well for the fish.
Now I rarely have to touch the needle valves at all. They stay rock steady for me.
Len