A thought on CO2 production with yeast

rrkss, doesn't protein production for enzmes and other stuff require nitrogen which can not be fixated from the atmosphere by Eukaryotes (i.e yeast) and which are obtained by yeast from proteins? I would think that this would be the major obsticale to growth without added protein to the solution.
 
You are right about yeast not fixating Nitrogen from the atmosphere but remember that they can still extract the Nitrogen from ammonia and out Nitrogen compounds or Ions. Being a unicellular fungi, they feed on nutrients and breed. In our example of yeast making CO2, they can extract their Nitrogen from the ammonia in Chloramines. Once the chloramines have finished oxydizing whatever they want, the ammonia is freed up and available for the yeast to convert into an amino group (NH2) from the (NH3) using a simple reaction and use that nitrogen to build their amino acids. Another thing is that any water harboring yeast will also eventually harbor prokaryotes with the ability of fix Nitrogen which the yeast can then feed on.
 
rrkss said:
ROFL!! Sometimes I forget that not everyone has a degree in Biology.
I have three degrees.

I have Honorary Degrees in: Yapping, Typing and Opinons

And I bet a dozen lazy monkeys that no one is gonna argue the above :D

Roan
 
Okay, results of the test run of adding H2O2 to a yeast solution producing CO2 is . . . .


inconclusive.


Why? I'll tell yah why!

Goldarned jello recipe doesn't work worth a crappola army latrine for me. TWENTY FOUR HOURS and NOW it starts to actually show some bubbles? Is that normal?

It's bubbling now. About 5 bubbles per minute :rolleyes:

So I'm going to keep an eye on the bubble count and wait until tomorrow morning when things are at a consistant rate.

Then I'll add some H2O2 and we'll see what happens.

Roan
 
I have read about the jello method. Please keep us tuned in, as I dont like singly supporting the sugar industry. I have actually considered buying 50lb bags at sams!!!
 
I'm using my regular sugar/water recipe for this now. I know this one works and how long it takes. It's already putting out CO2 nice and steady. In the morning I will do a bubble count and then add a small amount of H2O2 and see what happens.

I *will* tell you this though: before I tore down the jello I did throw a teaspoon of H2O2 in there -- just for kicks. The bubble rate increased from 15 bpm to 40 bpm and kept going until I tore it down an hour later. It does look like it does not kill the yeast and it does increase CO2 production.

However, this is inconclusive :) That's why I want to use my old recipe. I know how long the bottle lasts so I have a better guideline to use.

I'll keep you posted. I hope someone else is experimenting as well?

Oh, and it's pretty darn safe. I threw in at least a tablespoon of H2O2 just before I trashed the jello in the sink and it foamed a lot, but nothing blew :)

Roan
 
It might appear to be increasing bubble rate but I bet if you put some H2O2 and yeast without sugar into the bottle, you would get a nice fizz appearing like it increases bubbles. But we will have to see. I would more or less take the data after 24/48 hours as proof that this works or not.
 
What I intend to do is simply put the H2O2 in and bubble whatever gas is being produced into a fresh cup of tapwater.

If the pH lowers, it's CO2. If it doesn't, then it's not CO2.

Right?

Roan
 
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