Limited penetration of inhibitors into biofilms has been widely observed in previous investigations (de Beer et al., 1994; Stewart et al., 2001; Stewart et al., 2000; Xu et al., 1996). In those reports, the concentration profiles of hydrogen peroxide or chlorine in biofilms were directly measured with microelectrodes and limited penetration of the biocides into the biofilms was demonstrated. This phenomenon could be explained by several mechanisms: diffusional resistance of the biofilm matrix (Costerton et al., 1987), neutralizing reaction of the inhibitors with the biofilm constituents (van der Wande et al., 1989), adsorption of the inhibitors onto the biofilm constituents, and degradation of the inhibitors (Antizar-Ladislao and Galil, 2003).