I believe beneficial bacteria grows on almost any inert material that has surface area to harbor them underwater like the fish a much higher functioning organism with a brain and by mass requiring much more oxygen then bacteria are able to extract more then enough oxygen out of solution when normally saturated with 5-10 PPM oxygen. As far as canister filters go beneficial bacteria are in both the canister and the aquarium in quantities that are active to support the bio load and in a dormant stage to react to increases in bio load. I actually did an experiment where I stocked a new 120 gallon unscycled tank with 4 adult mollies and 2 Cory cats day one and used nothing more then an establish Magnum 350 canister filter off my 60 gallon heavily stocked tank (10 barbs, 5 tetra minor, 4 silver dollars, 2 Cory cats, one 7" Gobi, 2 betta M/F, 1 ADF, and 2 redwags) to establish it in 2 days. I wanted to prove rather then waiting weeks to cycle the 120 that the 120 would cycle almost immediately and the 60 tank would suffer no significant ammonia spike by swapping out magnum filters. The established magnum 350 filter from the cycled 60 tank for the uncycled new magnum 350 originally intended for the already stocked but uncycled 120 tank. The result was that both aquariums suffered only a .5 spike in ammonia almost simultaneously for 2 days before going to 0. In addition and immediately following the 0 reading on the 2nd day I purchased 4 medium size blood Parrots and placed them in the 120 tank and continued to stock weekly with no further spike in toxins. To me this was proof positive that beneficial bacteria given a healthy environment and what they need will colonize underwater in a canister as well as in the tank not only in sufficient quantity but in dormant numbers waiting to bio bloom at the fist opportunity as demonstrated in the 60 tank ability to recover without an established filter and a canister filter established for a much smaller aquarium ability to establish a tank twice the volume. But you see it makes perfect sense that they thrive underwater as our oceans and lakes are also not dependent on wet/dry filtration to grow organisms, in my very respectful opinion (IMVRO) 8^D.