A thread I started over in the general freshwater section got me thinking about this. I have a pH controller. It is hooked to a timer with my lights. When the lights go off so does it. When those go off my airstones come on. I run 8 or so hours of lights/CO2 and 16 hours of airstones everyday. There was no reason that I could see to be adding CO2 without light so I figured "why waste the CO2?" My natural tapwater pH here is around 7.6. My CO2 injector keeps it around 6.6 when it is running. The question is this--could the pH fluctuations caused by cycling off and on the CO2 be killing my fish? I've been running my setup this way for 4+ months with no problems until I had a unexplained nitrite spike last week. Now I'm wondering if I have been slowly damaging my fish all along by cycling the CO2 before I even had the nitrite spike that cost me about a dozen fish. Should I just leave my controller on 24/7 and let it add CO2 as necessary to keep my pH in the same range all the time?