well here's my co2 setup: pressurized with a 5 lb tank, reg, needle valve, into a diy power reactor at 1 bubble per second, 24 hours a day, on a 55 gal. You don't HAVE to shut it off at night, but I'm not pushing the limits of the co2 levels in my tank, it's between 15 and 20 ppm usually. Start out really slow, like a bubble ever 2/3 seconds, and test the water right after the light come on in the morning until you fond the rate that gives you about 20ppm at lights on. The amount of co2 used by the plants is actually very little compared to the amount that gasses off unless you have super high light on a tank STUFFED with plants.