I also agree, the bacteria in your filter should be enough. If you are worried though, you can put some of the old gravel (i wouldn't put all of it
) in a stocking or push some to the middle of the tank and then put the new substrate over it. I did this with my 55g when I changed from gravel to Eco Complete. I moved a couple handfuls of the old gravel to the middle (so you can't see it) and then put the Eco over it. I only had a HOB filter at the time, so I wanted to put my mind at ease by adding some of the old gravel.
