For most fish the answer is yes. In fact not feeding at all now and then is also a good idea.
Fry need to be fed 4-6 Times/day for optimal growth. The Other fish I feed more than once are discus. For a large fish they have a small digestive system and do better with several smaller feedings rather than one larger feeding. I am sure there are a few other fish that need a different feeding schedule, i just haven't experienced them yet.