Another possibility here, what about one of the gouramis? Is the remaining one aggressive? What type of gouramis are/were they? I only ask because I have seen first hand what a grumpy gold gourami can do to a tank full of neons and guppies. It is hellish.
Also, I noticed you originally posted nitrite and nitrate levels but no ammonia. What's the ammonia? Zero I hope.
One more thing, can you post an am and pm pH reading? KH? And your Co2 levels am and pm? Without the raw data, this thread could go on endlessly without getting you any answers.