I was told a drop per gallon, for a 72 gallon I would measure the drops in a med cup so you know how and don't have to count. The second tank I fishless cycled, I added the drop per gallon daily until I reached 5 ppm, then cut it in half and only added it a couple times a week, it worked great. My 1st tank I added double and I think it slowed the cycle down.