To be honest, I hardly ever wash my hands before or after tank cleaning... unless I have rinsed the filter pad and needed to rub it or if I have recently used scented hand lotion, cleaner or other chemicals. Unscented lotion (even recently applied) has never caused problems with my fish.
As for the siphon tube, I have a 40ft long tube (basically, a simpler, DIY python... but, I might add, about 4X cheaper) and sometimes I need to suck on one end to get it started... but most the time, I use the same method that sublime does. I put about a 1.5 ft section in the tank and let it fill with water, crimp one end and put my finger over the other, pull most the tubing out until it's about 1'' below the surface, remove my finger from the tip, then release the crimp... and away the water flows.
As for the siphon tube, I have a 40ft long tube (basically, a simpler, DIY python... but, I might add, about 4X cheaper) and sometimes I need to suck on one end to get it started... but most the time, I use the same method that sublime does. I put about a 1.5 ft section in the tank and let it fill with water, crimp one end and put my finger over the other, pull most the tubing out until it's about 1'' below the surface, remove my finger from the tip, then release the crimp... and away the water flows.