And yes, I conduct many, many tests.
So answer the direct question if you have done the test as you claim.
Over what range of NO3 is this true ...
independent of other factors?
You have a reference, the tank that is breeding the otto's, simply add the KNO3 and test to see.
It will not say if you have true independence, however, if you dose to say 40ppm for 2 weeks and the fish and breeding are still fine,
then you can say it is not due to NO3 over a range of say 0-40ppm for 2-3 weeks etc. Independent of other factors. You cannot control everything, but you can control NO3. This is much easier to test and rule things out this way.
I
cannot say that the shrimp and fish I have bred will not breed at 80ppm, because I have not had the NO3 that high. I have made errors and ended up dosing 160ppm + to a tank, others have done so as well due to mistakes, and no one noticed any fish deaths or problems afterward. These tanks also had ottos, but they where
not breeding. The other species included Apistos and dozen other species etc, many rare plecos and amano shrimp.
The question is not so much are they healthy and okay......I think that is a VERY easy test to falsify, the question is about breeding/brood production. So you need a breeder to test this hypothesis. If you are the only one to breed ottos, then only you will be able to test it by dosing KNO3 to 40ppm. Then you will know if it is due to KNO3 dosing.
We KNOW that the otto cats are fine at and well above 40ppm for NO3, whether or not they breed is a different question. Even then you could argue it's the genetic sock of your that are more sensitive than say mine are. Yes, CRS folks have tried this same argument with the high grades and there might be something to it. I cannot say. But as these test rule out more and more "causes", the back stepping becomes increasingly less likely as a generalization about NO3 being some detrimental thing. Inbred shrimp would seem far more sensitive.
The OP's question about risk to fish/livestock and KNO3 is dosing has been answered, There's no risk that anyone can show definitively.
They also have a method to calibrate their test method for verification.
Is the OP breeding otto cats?
Not likely, can you say definitively that 40ppm of NO3 from KNO3 is the cut off?
Not based on what you have said thus far. You have bred them, but that does not say anything over what ranges they are tolerant breeding wise. I know they are fine over a very large range, 0-80ppm over the long term for general health from KNO3 dosing alone.
Regards,
Tom Barr