I used to do 1/3 per day on my 60 gallon. Because my work load has been extremely heavy lately, I had not done any changes in 2 weeks. The fish get fed heavily and I was concerned I might need to do several changes today (finally got a lighter load). I tested the water for nitrites and nitrates this morning dreading what the test results would be. I use the API liquid test kits and my solutions are quite new. Surprisingly, both tests came back reading 0. To me that just means undetectable by the tests.
I thought about this surprise and realized the most likely reason was my deep sand bed. It is at least 4" in all areas. At times I see gas bubbles escaping from the sand bed. I do stir it gently every couple weeks and then it takes a few days for the bubbles to start again.
The sand bed must be working like a marine tank sand bed. The tank has 7 full grown angels (body size a bit larger than a silver dollar coin plus they are superveils), 4 cory cats and numerous MTS. Were it not for the deep sand bed the test results would have been disturbing.
Charlie