1. Do not use aeriation with daphnia.
2. 2 liter - 1 gallon coke bottles or jars work very well for culturing.
3. Feed morning and night.
4. A moderate amount of light can be used, but is not mandatory. They like a 16 hour light/ 8 hour dark photo period, but low light, and no light works too.
5. Daphnia like algae, yeast, and several other small particulate type feeds. We use Our Plankton Feed, Nanochloropus, yeast, spirulina, mixed Algae, digested trout chow and Selenastrum (to name a few) to feed our daphnia. For the Selenastrum, we feed until a light green color is in the culture water. For our plankton feed, we feed a few drops per liter, or around a 1/2 dropper full to a gallon.
6. Change water in all cultures once a week by dumping in a fine meshed net, or 53 micron screen sieve. Either serve up the caught up daphnia, or refloat them. Some may die due to this treatment. Then replace the water in your culture with new water. I usually just start new cultures by making up two new buckets/bottles, filling them up half way with new water, then adding a few drops of plankton feed, or algae, and pouring the older culture into the new ones. Then if I have left overs I feed them to the fish!
7. Keep sufficient cultures going if you are using the daphnia for feed. Their reproduction is sometimes sporadic. Fresh water will spure them to produce.
8. A decent culture can contain about 300+ daphnia per gallon of culture water.
9. *** SEE NOTE AT END OF THIS PARAGRAPH - If using larger containers than 1 gallon, something with a larger surface area works better than something with less surface area. For example a Kiddie pool does much better than a 55 gallon drum. Oxygen exchange is better with more surface area, and daphnia do not do well with bubbles! *** NOTE at END OF PARAGRAPH (sorry I just couldn't resist) I am currently have great success raising large amounts of Daphnia magna in 55 gallon drums. No air still, but I do stir in the plankton feed morning and night. I believe the results are due to temperature...see Ok, here's the plan!
bulletin board for last measurements on temp.. I will add some more here a bit later either tonight or tomorrow.... as I'm adjusting this info at the present moment (11-22-02).
10. Our Plankton Feed (see recipes for Plankton feeds I use) is good in a suspension of 1/2 tsp Plankton Feed to 6 ounces of water. Blend high for 3 minutes, and use a dropper. Starting cultures (low # of organisms,) take one drop of this mixture morning and night. As culture grows, double the feeding (2 drops

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11. Using a 5 mil pipette with a 14" 3/16" diameter flex aquarium tubing attached to the small end of the pipette allows me to quickly collect daphnia from a bowl so I can move them for a new water change.
12. daphnia can be siphoned into a 100 micron sieve as well, but use a bowl as a water buffer to prevent damaging them. They do bash around a bit, if one isn't careful.
13. When recieving a new culture of Daphnia or working with one which has sat awhile without water changes, then be sure to acclimate them to new water. They like soft to hard water, but need time to get used to extreme changes. This goes for Temperature differences, pH, and so forth...If I told you once, I've told you a 1000 times...
14. Oh, I think I forgot to mention: Like all cultures , temperature can make a big difference - most planktons do not do well when water temps rise above 90 degrees F (it may kill them) and 85 degrees F is pushing it!