Here is some other VERY important stuff even before starting your system or connecting the regulator to the tank.
BEFORE attaching a regulator to a filled CO2 tank, open the tank valve and let some CO2 shoot out. This will clear out any debris that may have lodged and is waiting to clog your regulator.
BEFORE attaching or detaching your regulator from your CO2 tank always adjust your working pressure down to zero. Putting on a filled CO2 tank with your working pressure more than zero could blow out your regulator dial. The only exception to this are the preset regulators. NEVER run your CO2 tanks empty. Running them empty can cause any settled debris to be blown into your regulator, clogging it. ALWAYS use a new washer between your regulator and the CO2 tank unless you have a perma washer.
And here is a great guide you should use to do a leak check on your regulator.
http://www.plantedtank.net/forums/e...check-co2-pressurized-system.html#post1390286
I also highly recommend doing a leak check at every hose connection. Also GO SLOW with adjustments. Be sure to use a good bubble counter. I also recommend a good drop checker in the tank. Make your adjustments easy and one bubble at a time. be sure when you set this up and get it running you have a WHOLE day you can spend with it and not give anything else your attention. Make your adjustment with 1 bps. let it set make sure your BPS doesnt change and is stable. Watch for a while. If your drop checker is still blue to dark green and the fish act fine then go up to 2bps and repeat. A drop checker can take up to 2 hours to change color. WATCH THE FISH FIRST as they will show you issues alot quicker then that. If you see your fish in the corner all huddled together and panting OR at the top of the tank gasping for air at the surface. Turn your needle valve down a little. Make sure you have a little agitation on the surface of the water. You should be able to have a lime green to yellow drop checker Fish acting normal. And if your light is high enough at the end of the day your plants should pearl.
What are you using to introduce the Co2 to the water? ie: Reactor, Ceramic diffuser, Atomic diffuser, needle wheel, other? This can make alot of difference on what your BPS is at. So will your plant load and how big the tank is. Gas exchange can make a little difference. Hows your circulation in the tank? It to is important for even distribution of Co2 in the water. Feel free to ask any questions.
BTW what regulator are you using?