The 100 ml bags are a poor fit in many, if not most, canister filter compartments, which have more area than the bags. Same goes for the AC 70 & 110 HOB's, where you need 2 to cover the available space, as you already know. As to re-charging Purigen, thousands have done it safely for many years, and there's no problem if rinsed very well in conditioned water, several times if need be. But if you wish to continue using the bag just once, and then replacing it, I'd suggest doing so before the beads get completely black, since at that point, they will have adsorbed all they possibly can and be useless.