Is this the same tank you cycled with 12 guppies, 2 of which survived? So the two guppies were in there when the cycle finished, then you added the Koi, the Cichlids, and the Pleco all at once? I'm not alluding to that shocking the system, I'm coming right out and saying it. And you are seeing the results now.No the Koi came in at the same time the Chichlids did. I did this to make sure there was no shock to the tank as you are elluding to.
The airstones are helping by effectively increasing the surface area of the tank. When the surface is rippled, the increased water to air interface area can exchange more gasses. Let the surface go calm and there isn't enough area for the exchange to happen fast enough. The fish get starved for oxygen and come to the top. After a while of this, they will come to the top and float belly-up. They are suffocating.I know that the air stones only help stir the water and thats why I don't understand the fish reaction to my turning off the air.
So you have added another filter other than the Fluval-3 you had in there? That filter would be adequate if the tank was stocked normally, but Koi change everything.knowing that it was going to require more changes and filtration hence the oversized filter and cleaning it every other week.
Everybody that has answered this post, and the others has told you the same thing... Maybe there's something to it? I'll be amazed if 50% of these fish make it until Thanksgiving. You really need to do something....