Test tube Beneficial Bacteria

Here are the results after the initial round in the anerobic chamber
The streak plate and the lawn plate from the Dwarf Puffer tank
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DP lawn
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as you can see in this one there is one morphology of bacteria present and proliferate in the the lawn, the streak is lacking due to error on my part.

Here is the streak and the lawn from the invert tank.
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Invert lawn
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as you can see there are many more morphologies on this plate. 6 in total
1. Orange smooth colony
2.White smooth colony
3. Peach smooth colony
4. White rough colony
5. Small yellow smooth colony
6. Pink(ish) smooth colony
I made a plate of each of these colonies to further isolate them.
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There is 8 in this picture because I did a streak of the DP slides for consistency



After talking to my professor he explained that the anaerobic chamber was to select for bacteria that are anaerobic and select against anything else in the water that needs oxygen to survive and could be considered a contaminant.
 
Yep, I agree with the above statements about the bacteria needing oxygen. The BB we have growing in our tanks tend to live in the most oxygen rich parts of our tank I.E. the filter becuase the water is constantly in motion and being oxygenated there. When you seed someones tank with media from an established tank, one of the most important things to remember is to make sure the media remains oxygenated so that all the BB won't die off before getting into the new seeded tank, or it won't work. Definitely needs oxygen to survive.
 
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