thanks blinkyBlinky said:AFAIK, CO2 levels can increase in the water if there is a lack of adequate circulation, a heavy film on the surface, or anything else that prevents the exchange of CO2/O2 at the surface. In regular conditions (circulating water, surface of the water being broken by airstone bubbles or filter outflow), I believe the CO2 in the water and atmospheric CO2 equalize, but I could be wrong.