On the last question first, Prime might possibly add chemicals that will affect test results, as Miguel earlier mentioned. A microbiologist friend of mine once told me that we don't always know exactly what chemicals may be in certain products, and some absolutely do cause inaccurate tests. Given my previous comment about using Prime, I certainly would not use it more than necessary to condition "new" water being added. Aside from anything else, Prime, like all conditioners, adds TDS (total dissolved solids) and Prime actually adds quite a bit, and this is something that soft water fish like cardinals do not at all appreciate. We think of "hard" water as meaning mineral hard, like calcium and such, but hard water also includes any TDS, and these arrive in all sorts of things from water conditioner, fish food, plant fertilizers, treatments, additives, etc. The fewer the better.Thanks Miguel for the link and help.
Byron thanks again. What really baffles me is that I didn't get readings of ammonia after my tank cycled, until my old tank leaked.
I am now realizing that the filter and filter media stood in tank water without oxygenation for several hours!
Also the gravel completely dried up too.
Would these be the cause of my Ammonia readings?
Would dosing Prime more than recommended (trying to save fish from the ammonia readings) also cause false readings?
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As for the ammonia from the substrate drying, I have heard this elsewhere. No direct experience, but someone else may be able to confirm. Same for the filter media. Nitrifying bacteria will survive longer than many realize in the absence of oxygen, ammonia, etc, and I've no idea just how long. This article may have more info for you:
http://www.practicalfishkeeping.co....r_bacteria?_Dream_on…&utm_content=html
Byron.