I run a Penguin 125 on my 20g long tank. According to the instructions, when the sponge gets all clogged up and water starts overflowing around the intake tube, you're supposed to change the sponge to a new one. But I'm wondering...does this remove a lot of the nitrifying bacteria, or are most of the bacteria left on the biowheel? It wasn't a problem the last time I changed the sponge, but the more I think about it, I just want to be sure.