Project Orange

Phyto Update 01-Nov-10

As you can see, the Nannochloropsis is taking hold.

The Dunaliella is a no go. Those spots you see in the pic are contaminates. Not sure how they wound up in here. Will be scrapping it and starting a fresh batch tomorrow.

Phytoplankton_110110.jpg

Phytoplankton_110110.jpg
 
Restarted the Dunaliella today. The starter culture is really light in color. Not really sure it is very viable. I doubled the amount of culture to start with to 20ml.

The Nanno continues to get greener. I noticed particles floating in this jug too but since it is growing, I'll leave this one alone. I'll have to try and determine when to split this one. I think it needs to mature for a few more days yet.

Should still be on target to test both salinities out.
 
Well my project just has all kinds of speed bumps in it. Forgot to update this.

Either I miscalculated the date or the ladies dropped their eggs. [scratches head]

Going by the first cycle, I thought I would have been good to go. The ladies lost the eggs a week earlier than expected. Soooo...as much as i didn't want to do it, I think what I will do next go around, will be to seperate the females after the first week of egg development. Didn't want to keep them isolated for so long.

Dunaliella culture still a no go. I'll be ordering fresh cultures today. Which reminds me that I need to check on the other two specimens to see if they are still viable as well. Yikes! Forgot about them two.

The Nannochloropsis has been split and two bottles now culturing. By next week I should be starting to refridgerate some for future use. I should also start looking to see if there are any reef keepers that may want a fresh source. ;)
 
Yeah...not a whole lot of information to go on so I'm building onto it as I go. Once I learn their traits and habits, I'll have that part down. Then the real tricky part starts.
 
Scrapped the Dunaliella. The second batch didn't take off either. So, needing a pure salt phyto, I started two new cultures: Isocrysis Galbana and Tetraselmis. Ordered a fresh culture of Dunaliella and will start it when it arrives. Not really sure I want three pure salt cultures going at one time but will think that over when the time comes. Brackish setup will be ready.
 
Phytoplankton's in the sea water are starting to grow. That's a good sign. Will be harvesting/storing the first batch of Nannochloropsis this weekend. If I read correctly, I can store it in the fridge for about a month. Not sure if I would keep it that long though. Will need to find some reef keepers that want some fresh cultures.

Phytoplankton_111810.jpg


No berried shrimps as yet but keeping an eye out. I'm thinking of picking up a couple other species since there will be an abundance of phyto. Haven't decided just yet. I know I would like to track down the Ninja shrimp to add to this project.
 
Ok....we're ready for the third attempt at this. I found a berried female today. Plan will be to keep her in the tank for 1 week and then isolate her. The question will be about other females that may get berried during this time.

All phytos are growing nicely. I will be able to conduct both brack and sea tests this round.

- crosses fingers -
 
So far we have two volunteers for this round of testing:

SunkistWithEggs_112210.jpg


This little lady is working over a plant floating up top:
SunkistWithEggs2_112210.jpg
 
AquariaCentral.com