That's the thing, BIO-Spira is supposed to cycle your tank as close to instantly as it can get. There may have been a small spike already while you were sleeping, eating, reading or whatever. If the BIO-Spira worked perfectly, then it could have already started converting. I would second what Gunnie already stated above
Gunnie said:Check for nitrates. That's when you will know the bio spira is working. Post your results here please!