For sure. It's like trying to get nitrate readings off a neon in a 55Not necessarily. The nitrate may be reading 0 ppm, but they may actually be so low that they are not able to be registered by the API kit which is only able to go as low as 5 ppm. The OP has a lot of water and a very light stocking right now.
Right now I'm most concerned with how the tank water is collected. There's no telling what the water is picking up along the roof. I'd set something up where you're getting direct rainfall and not any runoff.