I am getting conflicting advice about agitation / aeration at night. Also, some say to keep co2 on all day/night. If I dont need nighttime agitation, then I may not need it...
It appears that you have a solenoid with your regulator, right? I use a timer in conjunction with the solenoid to turn on my CO2 around a half-hour before the lights kick on, and then off when the lights go out. While dark, the plants don't really take up CO2, but rather they expel oxygen. If you then agitate the surface, and out-gas your CO2, you will have a harder time keeping your CO2 saturation, and also your pH stable.