Very interesting stuff going on here. I just wanted to pipe in with my experience. Last year I setup a 29G with play sand, and put more in than I planned. It ended up about 4" deep. The tank is densly planted with lowlight plants. It's stocked with white clouds, a couple bamboo shrimp, and a TON of snails. MTS, ramshorns and brigs. Over this last year or so that it's been setup, the more course grains have worked to the top, and the finer sediment has sunk to the bottom. So far, I'm yet to see a single black pocket. Maybe there are some in there somewhere, but they're not visible against the glass. No matter how long I go between water changes (sometimes a month or so) my water parameters are always spot on, with nitrate never going above 20, if that. When you consider there are hundreds maybe thousands of snails, including several brigs, I'd say it's a HEAVILY stocked tank. Still, it's by far one of the happiest, most self sufficient, thriving little ecosystem I've ever had. the plants seem to love the sand. I tried to uproot a willow hygro the other day, and finally jsut gave up LOL. I do have to admit though, whenever I do disturb the sand in any way, I follow it immediatly with a massive water change, just to be on the safe side! But the water has NEVER stunk.