Further info on Prime and the nitrogen cycle posted by another member.
http://www.aquariacentral.com/forums/threads/seachem-prime-and-test-readings-and-other-info.283725/
It looks like my previous information wasn't completely accurate, sorry about that!According to that post prime should protect your fish from the ammonia, even though your test will show it, for up to 48hrs and your bacteria can still use that ammonia to colonize.
Oh, and thank you, you have gone above and beyond when it comes to assisting others! you are absolutely amazing, and have been very helpful! It's greatly appreciated.